Details

Finger tip blood at 400x + cam zoom. Addition of PH agent was added (PH = ~4-5) & video view time starts at 30+ hours with 10+ hours of time lapse at 15s intervals. Entire video was sped up 10x for brevity, but a considerable length to the video was given for all to witness the gradual progression & to satisfy ones inquisitiveness. Slip cover was sealed using Vaseline.

A very defined String of Pearls spirochete was assumed to be going through blebbing formation & the next phase of dismantling & dispersion, yet it surprises us with a retraction of the individual beads or blebs as it reverts back to the original form factor and seemingly dissolves the granules into its body.

PH agents are a beautiful way to reveal these pathogens, as I now have a more comprehensive way to view the total load & infection. Even better than just with viewing blood standalone. The majority of items & even string-like objects in this video are not pathogenic, yet many can be seen appearing from time to time throughout the time lapse. Other SoP’s come out of RBC’s, besides the main one in focus of this video, as well as many other sizes and morphologies. This does not represent the best of what I have seen thus far, as PH buffering of blood allows a view not possible before in most cases.

THEORY ON WHY THIS HAPPENS:

I added one drop of buffering agent to the slide & then touched one drop of fingered blood right on top of the drop of agent. So drop to drop touch & then used the slip cover to have the mixture run along the one edge of the slip cover to then smear across. This makes for an uneven distribution, which is what I wanted since I am not sure what the OPTIMAL PH would have to be for maximum visualization of total spirochete load without compromising the spirochetes into cysts or dissolving the spirochetes altogether. So I figured it might be best approach for now.

The agent does not mix with blood uniformly & there are pockets of areas where PH is higher & pockets where PH is lower. Some areas the RBC’s can Lyse, depending on PH of buffer, & other areas will have partially disturbed rbc’s & yet other areas will have intact erythrocytes. The areas that have lysed rbc’s can further change the PH for adjacent areas to influence ph differently & disproportionately as the hemoglobin & intracellular contents spew into the plasma.

The PH of areas can become more basic (less acidic) over time & so my explanation of the reversing of the SoP formation is the gradual increase of PH (more alkaline) of plasma in this specific area over time (may not be true for other areas & all points on the slide). I have also witnessed one other phenomena with PH buffered blood & blebbing and so I use these two observations to propose that BLEBBING IS THE RESULT OF PH IN THE ENVIRONMENT IN THESE ATYPICAL FORMS. It would be nice to know what happens to the typical forms in ticks too.

So basically when an animal dies in real life or your blood dies on a slide, the blood environment becomes more acidic & it is this PH driving force that the spirochete detects as the unfavorable condition. It then takes the best protocol from millions of years of evolution & breaks up into many pieces (spores, aka granules, aka blebs, aka beads of string) for the most efficient dispersion.

I did not know reversion of SoP was possible until the buffer gave me clues & as I said coupled with another clue has allowed me to postulate this. When the PH buffer more evenly dispersed in the blood prep & as more rbc’s lysed & made the surrounding plasma less acidic, the SOP detected favorable conditions once again & reverted back.

Obviously more could be done with this information, but better to have a starting point rather to be guessing aimlessly & hopelessly.