Borrelia Burgdorferi Biofilm – Live Microscopy Video
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Eva Sapi, Ph.D., Associate Professor of Biology and Environmental Science at the University of New Haven, hosted my visit 2008. She collaborated with Dr. Alan MacDonald, retired pathologist and Borrelia career researcher, to discover and prove that Borrelia creates biofilms, as microbes have been doing for three billion years.
Using video microscopy, Dr. Sapi’s grad student David Luecke explains in this video how DNA from the Borrelia burgdorferi strain B31 “fluoresces” or glows an eerie green. Any green life forms you see in their various states originate from the B31 strain of Borrelia — one of 100+ strains known.
REMINDER: Most Lyme disease tests only test for ONE strain of Borrelia! Igenex, Advanced Labs and Spirostats will test for more than one strain, however.
Prior to Dr. Sapi’s pioneering efforts researchers dismissed Borrelia biofilms. As well, Borrelia biofilm would ruffle the feathers of the IDSA, NIH, CDC and (most) insurance companies, as it would spell out an obvious scientific syllogism for neuroborreliosis — known previously as chronic Lyme Disease.
Later, they learned that Sapi et al were correct — that the standard BSK media could be enriched with collagenase (which humans have abundantly) and this media proved to be ideal for growing spirochetal, slimy communities!
This video shows four different life forms alive in the community: spirochetes, cysts, granular and of course the biofilm community itself. Biofilms protect the microbial community from the immune system and antibiotics, which enable infections to live indefinitely inside us.
Hence, chronic Borreliosis (chronic Lyme disease).
Note: you may have seen this before in low resolution. I offered it to the producers of Under Our Skin years ago (free of charge). In this HD version, I included some amazing new high res AFM photos and additional explanations. Special thanks to David Luecke, who provided these slides, and of course to the Sapi-MacDonald team!
This video was produced by The Arthroplasty Patient Foundation. Please offer an any-sized, tax deductible donation at this link:
http://www.arthropatient.org/about/donate/
Our specific library of current intelligence on biofilms:
http://www.biofilmcommunity.org/
Our documentary on biofilms, Why Am I Still Sick, now available in French, Spanish, Simplified and Traditional Chinese:
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